The ACES (AthenaES Complete Expression System) Promoter Selection Kit
applies the principle that the overall expression rate can determine not only the
total accumulation level, but also the amount of soluble protein that is made. By
titering the promoter activity, one can fine-tune the expression level to meet the
specific needs of target protein production levels.
The ACES (AthenaES Complete Expression System) Signal Sequence Kit
provides a set of six expression vectors each with a different signal
sequence for protein export to the periplasmic space of E. coli
using either the SEC, SRP or TAT protein secretion pathway. Since the use
of a post-translational or co-translational export mechanism is protein-specific
and cannot be known a priori, this kit allows for the selection of
the translocation pathway best suited for a given recombinant protein.
The ACES (AthenaES Complete Expression System) YebF Protein Export
Kit makes use of the YebF protein to chaperone other proteins through the inner
and outer membranes of E. coli cells. This permits the secretion of
target proteins into the culture medium, simplifying downstream purification
as well as allowing for the expression of toxic proteins and proteins that
do not fold correctly in the E. coli cytoplasm.