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AthenaES™ Protein Expression Media
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ACES™ Promoter Selection Kit
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The ACES™ (AthenaES™ Complete Expression System) Promoter Selection Kit
applies the principle that the overall expression rate can determine not only the
total accumulation level, but also the amount of soluble protein that is made. By
titering the promoter activity, one can fine-tune the expression level to meet the
specific needs of target protein production levels.
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| Component |
Quantity |
| Plasmids |
| pAES25 |
10µg |
| Strain |
| JM109 Competent Cells |
2 x 200µL |
| Primers |
| Primer A |
250pmoles |
| Primer B |
250pmoles |
| Medium |
| Turbo Broth™ |
1L |
| Superior Broth™ |
1L |
| Power Broth™ |
1L |
| Hyper Broth™ |
1L |
| Glucose M9Y |
1L |
| Glucose Nutrient Mix |
21g |
| LB Miller Broth |
1L |
| Medium Supplements |
| Augmedium™ |
25mL |
| Inducer Solution A |
1mL |
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