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AthenaES™ Protein Expression Media
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ACES™ Signal Sequence Kit
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The ACES™ (AthenaES™ Complete Expression System) Signal Sequence Kit
provides a set of six expression vectors each with a different signal
sequence for protein export to the periplasmic space of E. coli
using either the SEC, SRP or TAT protein secretion pathway. Since the use
of a post-translational or co-translational export mechanism is protein-specific
and cannot be known a priori, this kit allows for the selection of
the translocation pathway best suited for a given recombinant protein.
This product encompasses patented technology. You must become a Registered User and
agree to a Non-Commercial Use License or a Commercial Evaluation License in order
to purchase or use this product. Click on the Link below to learn more about the License
Agreements and becoming a Registered User.
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| Component |
Quantity |
| Plasmids |
| pAES30 |
10µg |
| pAES31 |
10µg |
| pAES32 |
10µg |
| pAES33 |
10µg |
| pAES34 |
10µg |
| pAES35 |
10µg |
| Strain |
| JM109 Competent Cells |
2 x 200µL |
| Medium |
| Turbo Broth™ |
1L |
| Superior Broth™ |
1L |
| Power Broth™ |
1L |
| Hyper Broth™ |
1L |
| Glucose M9Y |
1L |
| Glucose Nutrient Mix |
21g |
| LB Miller Broth |
1L |
| Medium Supplements |
| Augmedium™ |
25mL |
| Inducer Solution A |
1mL |
| Inducer Solution B |
100mL |
| Secretion Enhancer Solution A |
60mL |
| Secretion Enhancer Solution B |
60mL |
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