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AthenaES™ Protein Expression Media
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ACES™ YebF Protein Export Kit
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The ACES™ (AthenaES™ Complete Expression System) YebF Protein Export
Kit makes use of the YebF protein to transport other proteins through the inner
and outer membranes of E. coli cells. This permits the secretion of
target proteins into the culture medium, simplifying downstream purification
as well as allowing for the expression of toxic proteins and proteins that
do not fold correctly in the E. coli cytoplasm.
This product encompasses patented technology. You must become a Registered User and
agree to a Non-Commercial Use License or a Commercial Evaluation License in order
to purchase or use this product. Click on the Link below to learn more about the License
Agreements and becoming a Registered User.
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| Component |
Quantity |
| Plasmids |
| pAES40 |
10µg |
| Strain |
| JM109 Competent Cells |
2 x 200µL |
| Antibodies |
| Anti-YebF Antisera |
0.5mL |
| Medium |
| Turbo Broth™ |
1L |
| Superior Broth™ |
1L |
| Power Broth™ |
1L |
| Hyper Broth™ |
1L |
| Glucose M9Y |
1L |
| Glucose Nutrient Mix |
21g |
| LB Miller Broth |
1L |
| Medium Supplements |
| Augmedium™ |
25mL |
| Inducer Solution A |
1mL |
| Inducer Solution B |
100mL |
| Secretion Enhancer Solution A |
60mL |
| Secretion Enhancer Solution B |
60mL |
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