AthenaES™ Protein Expression

ACES™ YebF Protein Export Kit

ACES™ (AthenaES™ Complete Expression System): YebF Protein Export Kit
The ACES™ (AthenaES™ Complete Expression System) YebF Protein Export Kit makes use of the YebF protein to transport other proteins through the inner and outer membranes of E. coli cells. This permits the secretion of target proteins into the culture medium, simplifying downstream purification as well as allowing for the expression of toxic proteins and proteins that do not fold correctly in the E. coli cytoplasm.

Plasmid pAES40

Component Quantity
Plasmids
pAES40 10µg
Strain
JM109 Competent Cells 2 x 200µL
Antibodies
Anti-YebF Antisera 0.5mL
Medium
Turbo Broth™ 1L
Superior Broth™ 1L
Power Broth™ 1L
Hyper Broth™ 1L
Glucose M9Y 1L
Glucose Nutrient Mix 21g
LB Miller Broth 1L
Medium Supplements
Augmedium™ 25mL
Inducer Solution A 1mL
Inducer Solution B 100mL
Secretion Enhancer Solution A 60mL
Secretion Enhancer Solution B 60mL
Order Here
0148-3 ACES™ YebF Protein Export Kit 1 kit $240.00
Kit Components: Available For Separate Purchase
0149-40 pAES40 10µg $150.00
0151-
JM109-C
JM109 Compentent Cells 2 x 200µL $55.00
0313-1 Anti-YebF Antisera 0.5mL $150.00
0152-1 Inducer Solution A 1mL $16.00
0152-5 Inducer Solution A >5 x 1mL $76.00
0153 Inducer Solution B 500mL $55.00
0154 Secretion Enhancer Solution A 500mL $55.00
0155 Secretion Enhancer Solution B 500mL $55.00
0104 Turbo Broth™ 500g $55.00
0105 Superior Broth™ 500g $58.00
0106 Power Broth™ 500g $48.00
0107 Hyper Broth™ 500g $41.00
0108 Glucose M9Y 500g $31.50
0103 LB Broth (Miller) 500g $46.00
0109 Glucose Nutrient Mix 150g $15.00
0123 Augmedium™ 100mL Stock $106.00
ACES™ YebF Protein Export Kit Application Manual (pdf)
ACES™ Signal Sequence and YebF Expression Systems
       Technical Brief
Download pAES40 Map and License Information (pdf)
Download pAES40 Plasmid Sequence (pdf)
Improved Expression of Recombinant Proteins In E. coli