AthenaES™ Specialty Proteins
I27O™ AFM Reference Protein
Bacterial Esterases
Gaussia Luciferase
Renilla mullerei Luciferase & Renilla reniformis GFP
Ptilosarcus Green Fluorescent Protein
Anti-NKX3.1 Antibody

Anti-NKX3.1 Polyclonal Antibody

For the detection of NKX3.1 protein in prostate cancer.
Anti-NKX3.1 Polyclonal Antibody
  • Immunohistochemistry
  • Immunoblots
  • Quantitative EIAs
Anti-human NKX3.1 is the IgG fraction from rabbit anti-sera generated by ­immunizing the animals with purified recombinant human NKX3.1 protein. The immunogen corresponds to the amino-terminal 153 amino acids of the human protein (1). The IgG fraction was prepared by Protein A affinity chromatography.

NKX3.1 is located on human chromosome 8p21.21. It is an ­androgen-­regulated homeodomain gene with the expression of the NKX3.1 protein ­predominantly localized to prostate epithelial cells (2,3).

1. Bethel, C. R. et al. 2006. Decreased NKX3.1 protein expression in focal prostatic atrophy, prostatic intraepithelial neoplasia and adenocarcinoma: association with Gleason score and chromosome 8p deletion. Cancer Res. 66:10683-10690.
2. Abate-Shen, C., Shen, M. M. and Gelmann, E. 2008. Integrating differentiation and cancer: the Nkx3.1 homeobox gene in prostate organogenesis and carcinogenesis. Differentation; research in biological diversity. 76:717-727.
3. Gruel, B. et al. 2010. NKX3.1 as a marker of prostatic origin in metastatic tumores. Am. J. Surg. Pathol. 34(8):1097-1105.
4. Guan, B., Pungaltya, P. et al. 2008. Ubiquitination by TOPORS regulates the prostate tumor suppressor NKX3.1. J. Biol. Chem. 283(8):4834-4840.
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0314 Anti-NKX3.1
Antibody		 1 mg IgG $300.00
Datasheet - 0314 Anti-NKX3.1
Gruel et al 2010
Anti-NKX3.1 Antibody Staining of Prostetic Adenocarcinoma
A soft tissue metastasis stained with H&E (A), NKX3.1 (B), PSA (C) and PSAP (D). Note the lack of staining with PSA, while NKX3.1 and PSAP still stain strongly. (Figure 4 from Gruel et al 2010. Am J Surg Pathol. 2010 August; 34(8): 1097–1105)