AthenaES™ Specialty Proteins

Anti-NKX3.1 Polyclonal Antibody

For the detection of NKX3.1 protein in prostate cancer.
Anti-NKX3.1 Polyclonal Antibody
  • Immunohistochemistry
  • Immunoblots
  • Quantitative EIAs
Anti-human NKX3.1 is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant human NKX3.1 protein. The immunogen corresponds to the amino-terminal 153 amino acids of the human protein (1). The IgG fraction was prepared by Protein A affinity chromatography.

NKX3.1 is located on human chromosome 8p21.21. It is an ­androgen-­regulated homeodomain gene with the expression of the NKX3.1 protein ­predominantly localized to prostate epithelial cells (2,3).
Mouse nkx3.1 is an androgen-regulated homeodomain gene with the expression of Nkx3.1 protein predominantly localized to the prostate epithelial cells(5). Anti-mouse Nkx3.1 antibody is the IgG fraction from rabbit anti-sera generated by immunizing the animals with purified recombinant mouse Nkx3.1 protein. To improve the specificity of the antibody, only the N-terminal domain of mouse Nkx3.1 was produced as immunogen. The IgG fraction was prepared by protein A affinity chromatography. IgG specific to mouse Nkx3.1 was further purified using an affinity matrix produced by cross-linking the immnunogen to agarose.

1. Bethel, C. R. et al. 2006. Decreased NKX3.1 protein expression in focal prostatic atrophy, prostatic intraepithelial neoplasia and adenocarcinoma: association with Gleason score and chromosome 8p deletion. Cancer Res. 66:10683-10690.
2. Abate-Shen, C., Shen, M. M. and Gelmann, E. 2008. Integrating differentiation and cancer: the Nkx3.1 homeobox gene in prostate organogenesis and carcinogenesis. Differentation; research in biological diversity. 76:717-727.
3. Gurel, B. et al. 2010. NKX3.1 as a marker of prostatic origin in metastatic tumores. Am. J. Surg. Pathol. 34(8):1097-1105.
4. Guan, B., Pungaltya, P. et al. 2008. Ubiquitination by TOPORS regulates the prostate tumor suppressor NKX3.1. J. Biol. Chem. 283(8):4834-4840. 5. Bieberich, C. J., Fujita, K., He, W. W., and Jay, G. (1996). Prostate-specific and androgen-dependent expression of a novel homeobox gene. J Biol Chem 271, 31779-31782.
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0314 Anti-human NKX3.1
1 mg IgG $350.00
0315 Anti-murine NKX3.1
30 μg Affinity Pure $350.00
Datasheet - 0314 Anti-NKX3.1
Datasheet - 0315 Anti-murine NKX3.1
Gurel et al 2010
Anti-NKX3.1 Antibody Staining of Prostetic Adenocarcinoma
A soft tissue metastasis stained with H&E (A), NKX3.1 (B), PSA (C) and PSAP (D). Note the lack of staining with PSA, while NKX3.1 and PSAP still stain strongly. (Figure 4 from Gurel et al 2010. Am J Surg Pathol. 2010 August; 34(8): 1097–1105)