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AthenaES™ Protein Expression Media
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Augmedium™
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- Medium Additive
- Dramatically Increases Level of Expression
- Increases Fraction of Soluble Protein
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Augmedium™ is a medium additive which conditions cells prior to
induction of recombinant protein expression. This pre-induction conditioner
increases the level of chaperone proteins which can improve the fraction of
product that accumulates as soluble protein. Augmedium™ is specifically
intended for use with strains in which the target protein accumulates as an
insoluble aggregate. Augmedium™ is supplied as a powder for preparation
of 50x concentrated stock solutions. It is available in 100g and 500g amounts.
The graph on the right shows the level of expression of LypA with increasing
levels of Augmedium™. The amount of activity of LypA directly correlated
with the concentration of Augmedium™ and showed a significant increase of
LypA accumulation. IGP is the isogenic parent strain.
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