| Bioline™ Chromatography Systems |
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HR Chromatography Columns and Accessories
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- Chemically inert and biocompatable materials
- Pressure resistant to 1,450 psi (100 bar)
- Axial compression with adjustable bed height
- Uniform flow distribution
- Thermal jacket for temperature control
- Easy to exchange PEEK or PTFE frits and tubing>
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Bioline™ high resolution glass columns offer pressure stability up to 1,450 psi (100 bar) and a thermostatic jacket for temperatures between 0°C and 60°C, making them suitable for a wide range of bio-chromatography applications. Symmetrical “Uniform Flow” distributors on both ends make reverse flow (up/down flow) possible and minimize peak broadening. The column hardware design allows for easy exchange of frits and compression of the gel bed using the integrated compression screw. Moreover, the columns are equipped with an integrated quick release lever and can be closed with just a few thread rotations.
Scalable: Bioline™ glass columns can easily be adapted to different separation tasks, because they are available in 3 different inner diameters (10, 20, 30 mm), each available in lengths of 30, 60, or 100 cm. For adjusting to the column bed height, the variable adaptor offers a maximum displacement of 120 mm. The column can be equipped with up to two adjustable adaptors.
Modular: All column components – including the glass column and the adjustable and fixed adaptors – are also available individually. The tubing is not fixed to the column and is completely exchangeable, allowing you to choose between 1/16” Teflon® (PTFE) or PEEK tubing.
Durable: The robust, pressure resistant design of the filling reservoir and finger-tight adaptors ensure safe handling, ease-of-use and longevity.
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| Order Here |
| Complete Column Kits - Glass tube, thermal jacket, fixed and variable adapter |
| 10301 |
10mm x 30cm |
15 ml |
$1,326.00 |
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| 10302 |
20mm x 30cm |
60 ml |
$1,326.00 |
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| 10303 |
30mm x 30cm |
140 ml |
$1,492.00 |
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| 10304 |
10mm x 60cm |
40 ml |
$1,658.00 |
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| 10305 |
20mm x 60cm |
150 ml |
$1,658.00 |
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| 10306 |
30mm x 60cm |
350 ml |
$1,658.00 |
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| 10208 |
20mm x 100cm |
280 ml |
$1,624.00 |
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| 10309 |
30mm x 100cm |
636 ml |
1,823.00 |
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| 10300 |
6 Column Set |
See below |
$9,175 |
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| Column Accessories |
| Columns and Thermal Jackets |
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| 10310 |
10mm x 30cm |
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$600.00 |
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| 10311 |
20mm x30cm |
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$600.00 |
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| 10312 |
30mm x 30cm |
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$600.00 |
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| 10313 |
10mm x 60cm |
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$480.00 |
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| 10314 |
20mm x 60cm |
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$480.00 |
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| 10315 |
30mm x 60cm |
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$630 |
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| 10316 |
10mm x 100cm |
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$579.00 |
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| 10317 |
20mm x 100cm |
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$579 |
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| 10318 |
30mm x 100cm |
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$745.00 |
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| Column Adapters |
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| 10319 |
Adjustable Adapter |
10 mm |
$580.00 |
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| 10320 |
Adjustable Adapter |
20 mm |
$580 |
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| 10321 |
Adjustable Adapter |
30 mm |
$580.00 |
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| Accessories |
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| 10322 |
1L Filling Funnel |
10, 20 and 30 mm |
$789.00 |
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| 10323 |
PEEK tubing |
1/16" |
$51 |
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| 10324 |
Coupling and Fittings |
Red |
$17.00 |
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Columns and Accessories |
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| Technical Specifications: |
| Technical Data |
Wetted Materials |
Specification |
| Dimensions(ID x length): |
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10 mm x 100 cm, 20 mm x 100 cm, 30 mm x 100 cm
10 mm x 60 cm, 20 mm x 60 cm, 30 mm x 60 cm
10 mm x 30 cm, 20 mm x 30 cm, 30 mm x 30 cm
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| Max. height of gel bed |
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Column length minus 10 cm
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| Max. pressure: |
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1 cm ID column 10 MPa
2 cm ID column 10 MPa
3 cm ID column 8 MPa
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| Temperature range: |
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0 – 60°C |
| Column tube: |
borosilicate glass |
precision glass tube, resistant against buffers and acids as well as HPLC solvents; not for use with hydrofluoric or phosphoric acid, nor alkaline substances |
| Cooling jacket: |
PMMA (poly(methyl methacrylate), Plexiglas®) |
for use with water + 10 % methanol (little corrosion with 100 % methanol) |
| Adaptor: head and flow distributor: |
PEEK (polyetheretherketone) |
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| Adaptor shaft: |
stainless steel(1.4404, 1.4541, and 1.4301) |
stainless steel, not in contact with solvents |
| Adaptor frits: |
Ø 10 mm: PEEK, 10 µm
Ø 20 mm: PEEK, 10 µm
Ø 30 mm: PTFE, 5 – 9 µm
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PEEK: resistant against buffers and acids as well as HPLC solvents
PEEK: resistant against buffers and acids as well as HPLC solvents
PTFE: resistant against buffer, methanol and acetone, do not use with acetonitrile
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| Capillaries, nuts and ferrules: |
1/16” PEEK |
resistant against buffers and acids as well as HPLC solvents |
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| Bioline™ and Biofox™ are Trademarks of Knauer GmbH, Berlin, Germany |
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