AthenaES™ Cell Culture Media

FNC Coating Mix®

Serum-Free Media
FNC Coating Mix®: Specially formulated reagent that dramatically increases attachment of cells to plast growth substratum.
  • Dramatically increases attachment of cells
  • Complete serum-free medium
  • Used by major cancer research centers
FNC Coating Mix® is a specially formulated reagent that dramatically increases the attachment of cells to the plastic substratum used for culturing mammalian cell lines. Increased cell attachment facilitates and encourages growth in adherent cell cultures. Many fastidious cell types such as human prostate and breast epithelial cells will attach and grow more effectively on FNC-coated plastic surfaces when cultured in the appropriate serum-free medium.

The photograph to the right shows the difference in monolayer development with and without FNC Coating Mix®. BRFF-55T prostate cancer cells were grown in HPC1™ medium at the same seed concentration for 3 days at 37°C, 5% CO2. The flask shown on left was pre-coated with FNC Coating Mix™ whereas the flask on the right was not pre-treated. Through image analysis, there was approximately 85% greater monolayer development in the flask coated with FNC than in the non-treated flask.
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0407 FNC Coating Mix® 50mL $84.50
0407H Human FNC Coating Mix® 50mL $136.00
Storage Store at 4°C, should not be frozen
Stability at 4°C 4 - 6 weeks once opened
pH 7.3 ± 0.2
Osmolality 270 - 300 mOsM
Effect of FNC Coating Mix® on Monolayer Cell Cultures
Serum-Free Media Technical Brief
FNC Coating Mix® Data Sheet
FNC Coating Mix® MSDS