AthenaES™ Specialty Proteins

Gaussia Luciferase

Gaussia luciferase is a luminescent protein with quantum yields higher than the luciferases from Renilla sp. and firefly
  • ATP-Independent
  • Brightest Fluorescent Proteins
  • Stereoselective
  • Smallest luciferase to date
Protein Analysis Amino Acid(s) Frequency
Length 185 aa Charged (RKHYCDE) 33.51
Molecular Weight 19,899 Acidic (DE) 12.97
Molar Extinction Coefficient 8,290 Basic (KR) 12.97
Isoelectric Point 6.88 Polar (NCQSTY) 21.08
Charge at pH 7 -0.15 Hydrophobic (AILFWV) 35.68
C Cys 5.95 (11)
Gaussia luciferase is a luminescent protein with quantum yields higher than the luciferases from Renilla sp. and firefly. It is ATP-independent, requiring only coelenterazine and O2 as substrates. Gaussia's high quantum yields make it particularly useful in immunoassays.


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0308-1 Gaussia luciferase 1mg by quotation
0308-2 Gaussia luciferase 300µg by quotation
Oxidation of coelenterazine by Gaussia luciferase to yield light
The above diagram depicts the oxidation of coelenterazine by Gaussia luciferase to yield light. Coelenterazine-utilizing luciferases, such as Gaussia luciferase, do not require accessory high-energy molecules such as ATP for their signal. Gaussia luciferase is the brightest of all luciferase enzymes, which makes it superior to other luminescent proteins in assays requiring very low detection limits.
Gaussia Luciferase Data Sheet