AthenaES™ Protein Expression Media

Glucose M9Y

Glucose M9Y is Animal-Product-Free
GlucoseM9Y™: Provides higher yields than traditional formulation of glucose M9
  • Provides higher yields than traditional glucose M9
  • Supplemented with Glucose Nutrient Mix
  • Available in Animal-Product-Free
Glucose M9Y is an APF Certified™ minimal media formulation supplemented with yeast extract for the cultivation of E. coli. It is based on M9 salts with a pH of 6.9 ± 0.2 and employs glucose as the carbon source.1 Alternative carbon sources may be substituted in the powder version if desired. Buffering is provided by a sodium-potassium phosphate system. Ammonium chloride provides a nitrogen source. Yeast extract is added to improve the protein production capacity of the medium and to permit cultivation of auxotrophic strains by supplying a source of amino acids, vitamins and minerals. Our formulation differs from the traditional formulation of glucose M9, which employes casamino acids. Our experience has shown that yeast extract typically gives higher yields of recombinant proteins than casamino acids. Glucose M9Y powder is supplied with a separate Glucose Nutrient Mix.
1 Anderson, E. H. 1946. Growth requirement of virus-resistant mutants of Escherichia coli strain B. Proc. Natl. Acad. Sci. USA 32:120-128.
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0108 Glucose M9Y 500g $32.00
0108-S Glucose M9Y Singles 1L Mix (10) $32.00
0119 Glucose M9Y Liquid 500mL $24.00
0109 Glucose Nutrient Mix 150g $16.00
Glucose M9Y Powder MSDS
Glucose M9Y Liquid MSDS
Improved Expression of Recombinant Proteins In E. coli