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AthenaES™ Protein Expression Media
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Glucose M9Y
Glucose M9Y is Animal-Product-Free
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- Provides higher yields than traditional glucose M9
- Supplemented with Glucose Nutrient Mix
- Available in Animal-Product-Free
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Glucose M9Y is an APF Certified™ minimal media formulation
supplemented with yeast extract for the cultivation of E. coli.
It is based on M9 salts with a pH of 6.9 ± 0.2 and employs glucose
as the carbon source.1 Alternative carbon sources may be
substituted in the powder version if desired. Buffering is provided by
a sodium-potassium phosphate system. Ammonium chloride provides a nitrogen
source. Yeast extract is added to improve the protein production capacity
of the medium and to permit cultivation of auxotrophic strains by supplying
a source of amino acids, vitamins and minerals. Our formulation differs from
the traditional formulation of glucose M9, which employes casamino acids.
Our experience has shown that yeast extract typically gives higher yields
of recombinant proteins than casamino acids. Glucose M9Y powder is supplied
with a separate Glucose Nutrient Mix.
We produce several dry powder bacteriological media products including: two standard media found in published methodologies (LB Broth and Glucose M9Y), and four proprietary media formulations, sold under the trade names Turbo Broth™, Superior Broth™, Power Broth™ and Hyper Broth™
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1 Anderson, E. H. 1946. Growth requirement
of virus-resistant mutants of Escherichia coli strain B. Proc. Natl.
Acad. Sci. USA 32:120-128.
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