AthenaES™ Protein Expression Media

Hyper Broth™

Hyper Broths is Animal-Product-Free
Hyper Broth: Ideal for fermentation cultures and feed-batch applications, Cell yields typically 4 to 5 times that of LB Broth
  • Cell yields up to 5 times higher than LB Broth
  • Ideal as fermentation culture base
  • Animal-Product-Free
Hyper Broth™ is an APF Certified™ proprietary media formulation developed by AthenaES™ for improved expression of recombinant proteins in E. coli. The medium is a complex, rich formulation which supplies amino acids, vitamins, and glucose at high levels. This medium yields the highest level of biomass of all AthenaES™ media. The medium is buffered at pH 7.3 ± 0.2 and has the highest buffering capacity of Athena's Expression Media, which permits high glucose levels. For expression of recombinant proteins, induction should be done at cell densities three times that of LB Broth. Cell yields are typically 5 times that of LB Broth depending on the strain. Plasmid copy numbers are significantly higher than they are for LB Broth. This medium is suitable as the base for fermentation cultures and is excellent for feed-batch applications. Biomass yields will depend on the process and the strain employed. Hyper Broth™ powder is supplied with a separate Glucose Nutrient Mix.
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0107 Hyper Broth™ 500g $44.30
0107-S Hyper Broth™ Singles 1L Mix (10) $44.30
0118 Hyper Broth™ Liquid 500mL $12.40
0109 Glucose Nutrient Mix 150g $16.50
Hyper Broth™ Powder MSDS
Hyper Broth™ Liquid MSDS
Improved Expression of Recombinant Proteins In E. coli