| AthenaES™ Specialty Proteins |
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I27O™ AFM Reference Protein
For Force-Extension Experiments
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- Reference for force extension experiments
- 8 saw-tooth extension curves
- Unique durable elasticity
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I27O™ is a unique recombinant polyprotein used for the calibration of and as
a reference for force extension experiments using atomic force microscopes. It
is supplied in a ready-to-use solution. The protein is composed of 8 repeats of
the Ig 27 domain of human titin. Single molecule measurements give up to eight
saw-tooth force-extension curves with the specifications below. The protein has
durable elasticity that allows for repeated unfolding and refolding.
The graph on the right demonstrates the unfolding and refolding cycles
of an I21RS8 protein probed with a double-pulse protocol. The protein
is first stretched to count the number of domains that unfold, N-total, (a-c, top traces),
and then it is relaxed to its initial length. A second extension after a delay, Δt,
measures the number of refolded domains, N-refolded (a-c, bottom traces).
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