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PDQ™ Protease Assay
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- Detection of nanogram quantities
- Quantitative or Qualitative Applications
- No Centrifugation Necessary
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The PDQ™ Protease Assay is a unique colorimetric assay used to detect protease
activity in aqueous samples. The proprietary substrate responds to a wide range of
proteases including serine, metallo, aspartate and cysteine proteases such as collagenase,
proteinase K, papain, pepsin, bromelin, ficin, trypsin and chymotrypsin. PDQ™
can be used with just a few simple steps to measure protease activity and requires no
centrifugation. The substrate is a cross-linked matrix containing protein substrate
and a dye-protein conjugate. Protease activity is detected spectrophotometrically
with increasing optical density proportional to increasing enzyme activity and can
detect nanogram quantities. Each kit is supplied with a trypsin solution for generating
standard curves (BAEE equivalent units). PDQ™ is supplied in 48 ready-to-use plasict vials.
The graph on the right shows the time-course degradation of the PDQ™
Protease Assay substrate by papain, chymotrypsin, proteinase K, and collagenase. Duplicate
reactions were incubated at 37°C and 0.2 N NaOH was added to the vials to stop the
reaction at the indicated times. The absorbance at 450nm was measured by transferring the
reaction mixture to spectrophotometric cuvettes. The results of this experiment show
the increased absorption over time due to protelytic degradation of the substrate, and
the ability of the assay to detect a wide range of proteases.
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