|
AthenaES™ Protein Expression Media
|
|
|
|
|
Power Broth™ & Power Prime Broth™
Prime Broths are Animal-Product-Free
|
|
- Cell yields up to 4 times higher than LB Broth
- Rich nutrient base
- Available in Animal-Product-Free
|
|
|
Power Broth™, Power Prime Broth™, and Power Prime-olate™ are
proprietary media formulations developed by AthenaES™ for improved expression of
recombinant proteins in E. coli. These media are composed of a complex, rich mixture
of amino acids, vitamins, and a carbon source at higher levels than those of Terrific Broth. The media
are buffered at pH 6.8 ± 0.2. Power Prime™ and Power Prime-olate™ are
APF Certified™.
For expression of recombinant proteins, induction should
be done at cell densities three times that of LB Broth. Cell yields are typically 3 to 4 times
that of LB Broth in shake flask cultures depending on the characteristics of the strain.
Several reports suggest that Power Broth™ and Power Prime Broth™ can increase
the amount of soluble protein accumulated for otherwise insoluble proteins. This medium is
suitable as the base for fermentation cultures. Biomass yields will depend on the process and
the strain employed.
|
|
|
|
My Account
