| AthenaES™ Cell Culture Media |
|
|
|
|
SFM Screening Kit
Serum-Free Media
|
|
- Samples of 5 of the AthenaES™ Serum-Free Cell Culture Media
- Includes FNC Coating Mix®
|
| Storage |
Store at -80°C |
| Stability at 4°C |
4 - 6 weeks |
| pH |
7.3 ± 0.2 |
| Osmolality |
270 - 300 mOsM |
|
The SFM Screening Kit is intended for researchers seeking to identify the most appropriate
SFM formulation for a specific cell type. The kit contains a 100mL sample of each of five
serum-free media including: BRFF-BMZERO™, BRFF-EPM2™, BRFF-P4-8F™, DMEM/F12,
and IMDM along with 25mL of FNC Coating Mix® for dramatically increasing cell attachment
to the plastic growth substratum. Each of the media is complete, ready-to-use.
The cell photographs on the right are of a SFM Screening Kit experiment to determine the optimum
serum-free media for the production of the prostate cancer cell line 267-B1. The cultures were
seeded with 2 x 106 cells/mL into each of the 5 25cm2 tissue culture flasks
pre-coated with FNC Coating Mix® and containing the following media: BMZERO™, DMEM/F12,
EPM2™, P4-8F™ and IMDM. The cells were grown for 24 hours at 37°C at 5% CO2.
The photographs (20x magnification) show the difference in cell growth between the different media.
The cells in the BMZERO™ media yielded a more rapid monolayer development than the other media
with the least growth in IMDM. These results demonstrated that BMZERO™ is the optimum medium
for the growth of 267-B1 cells.
|
|
|
|
My Account
