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AthenaES™ Protein Expression Media
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Superior Broth™ & Superior Prime Broth™
Prime Broths are Animal-Product-Free
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- Cell yields up to 4 times higher than LB Broth
- Highest plasmid copy numbers
- Rich nutrient base
- Ideal for large-scale plasmid preparation
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Superior Broth™, Superior Prime Broth™, and new Superior Prime-olate™,
are proprietary media formulations developed by AthenaES™ for improved expression
of recombinant proteins in E. coli. The formulations
are complex, supplying a nitrogen source, vitamins, and moderate glucose
levels. The media are buffered at pH 7.2 ± 0.2 to prevent acidification
of the medium during metabolism of the glucose. Expression of recombinant proteins
should be induced at cell densities twice that of LB Broth. Cell yields are typically
3 to 4 times that of LB Broth. Superior Broth™ shows the highest plasmid copy
numbers among the AthenaES™ family of media. This medium is an excellent choice
for large-scale plasmid preparations. Biomass yields will depend on the process
and the strain employed.
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