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I27O™ AFM Reference Protein Data Sheet

Catalog No. 0304

Product Description

I27O is a 94 kDa synthetic polyprotein composed of eight repeats of the I27 domain of human titin. It is used for the calibration of and as a reference for force extension experiments using atomic force microscopes. It is supplied in a ready-to-use solution. Single molecule measurements give up to eight saw-tooth force-extension curves with the specifications below. The protein has durable elasticity that allows for repeated unfolding and refolding.

Product Specifications
Protein 100g
Shipping shipped on dry ice
Long-Term Storage 10 years at - 80C
Aliquot to avoid repeated freezing and thawing.
Short-Term Storage 1 month at 4C
Formulations In SSI Buffer: 50 mM Na2HPO4, 300 mM NaCl,
20 mM imidazole, pH 8.0
Technical Information (force extension measurements)
Distance between peaks 24.1 0.34nm
Force Peaks 204 26pN
Persistence Length 0.30 0.07nm
Contour length 24.1 0.34nm

Instructions for Use

1. Dilute the stock protein solution to a concentration of 100g/ml in PBS pH 7.3 buffer and apply the sample to freshly evaporated gold coverslips.
2. Attach coverslips to the piezoelectric positioner stage.

The I27O was engineered with an amino-terminal hex-His peptide sequence and carboxy-terminal di-Cys. This confi guration is hypothesized to allow the protein molecules to attach to the coverslip at the carboxy-terminal leaving the amino-terminus free to attach to the silicon nitride cantilever tip, when the tip is brought in close proximity to the absorbed protein. It is this model which is believed to account for the high frequency (>50%) of observing all eight domain extensions after each retraction as well as the ability to perform extension and retraction experiments on a single molecule.

Material Safety Data

FOR RESEARCH USE ONLY. NOT INTENDED OR APPROVED FOR HUMAN, DIAGNOSTICS OR VETERINARY USE. Do not ingest, swallow or inhale. Do not get in eyes, on skin, or on clothing. Wash thoroughly after handling. For complete safety information see full Material Safety Data Sheet.


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