Detergent Screening Kit Data Sheet
Catalog No. 0601
The AthenaES™ Detergent Screening Kit is intended for use in protein refolding applications. Detergents have been shown to aid in the protein refolding process of both native and recombinant proteins. Numerous observations have demonstrated that some proteins will fold into their correct structure in the presence of detergent. However, no single detergent has proved to be suitable for all proteins. This requires that the best detergent for any given protein be determined empirically.
Athena’s Detergent Screening Kit provides researchers with 6 common detergents (10mL each), which have been shown to affect protein refolding, for determining the optimum detergent for a given protein refolding scenario. The kit permits the identification of the best available detergent for a given target protein in a rapid screening format. Individual detergents can also be purchased in 100mL quantities.
Detergents are usually used as a step in the protein refolding process, often in conjunction with cyclodextrins. The detergent binds to the protein in the dilution stage to form a detergent-protein complex which prevents aggregation of the protein. A binding agent, such as cyclodextrin, can then be used to strip away the detergent from the protein, allowing it to properly refold. For this step in the protein refolding process, AthenaES™ offers a Cyclodextrin Screening Kit, as well as individual cyclodextrins.
Instructions for Use
The following algorithm is recommended when screening detergents for their utility in refolding a specific protein:
Material Safety Data
FOR RESEARCH USE ONLY. NOT INTENDED OR APPROVED FOR HUMAN, DIAGNOSTICS OR VETERINARY USE. Do not ingest, swallow or inhale. Do not get in eyes, on skin, or on clothing. Wash thoroughly after handling. For complete safety information see full Material Safety Data Sheet.