FNC Coating Mix® Data Sheet
Catalog No. 0407
FNC Coating Mix® is a specially formulated serum-free tissue culture reagent
containing fibronectin, collagen and albumin that is used to enhance the attachment
of adherent cells to plastic flasks or microplates. The unique formula creates
an extracellular matrix that dramatically increases the rate of cell attachment
to any plastic substratum. The matrix accelerates monolayer formation, especially
when fastidious cell types such as human prostrate and breast epithelial
cells are being propagated. Mammalian cell cultures will attach and grow more
effectively on FNC-coated plastic surfaces when cultured in the appropriate
serum-free medium. AthenaES™ off ers several diff erent serum-free media that
when used in conjunction with FNC Coating Mix® increase the growth of cell
||This product is shipped with a cold pack. DO NOT FREEZE
||Store at 4°C
||2 years at 4°C
||7.1 - 7.4
||280 - 300 mOsM
Instructions for Use
To coat the growth surface, add 0.2 mL FNC Coating Mix® per square centimeter of
surface area of the culture vessel (For Example: use 5 mL
for a T-25 flask). The surface should be completely
covered with a layer of liquid.
Incubate for 30 seconds and remove the coating mix with a pipet.
Residual FNC Coating Mix® will not adversely affect the viability of
the cells to be plated. Do not return any “used” coating mix to
the original container of FNC. This will compromise the performance of the product.
The coating can be done immediately
before seeding the cells or up to one hour beforehand.
Material Safety Data
FOR RESEARCH USE ONLY. NOT INTENDED OR APPROVED
FOR HUMAN, DIAGNOSTICS OR VETERINARY
USE. Do not ingest, swallow or inhale. Do not get in
eyes, on skin, or on clothing. Wash thoroughly after
handling. For complete safety information see full
Material Safety Data Sheet.