Renilla reniformis Green Fluorescent Protein Data Sheet
Catalog No. 0310
Product Description
Renilla reniformis (sea pansy) is a species of soft coral that is characterized by a green
fluorescence. The green fluorescent protein obtained from this organism exhibits
nearly symmetrical excitation and emission peaks with a relatively small Stokes shift.1
This is in contrast to the more commonly employed GFP from Aequorea victoria which
has two wavelengths at which excitation occurs, and a broader emission spectra. The
monomer extinction coefficient for Rr-GFP is about 5.5-fold higher than the Aequorea
GFP which, in conjunction with a slightly higher quantum yield, produces a brighter
fluorescence. Recombinant Renilla reniformis GFP has the same level of fluorescence as
the native protein.2
| Product Specifications |
| Protein |
1mg (0310-1), 250µg (0310-2) |
| Long-Term Storage |
2 years at - 80°C
Aliquot to avoid repeated freezing and thawing. |
| Short-Term Storage |
1 month at 4°C |
| Formulations |
In: 10mM Na2HPO4, 140mM NaCl, 2 mM KH2PO4, 3mM KCl,
20% glycerol, pH 7.6 |
| Molecular Weight |
26kDa by SDS- PAGE
22kDa |
| Technical Information |
| Length |
233 aa |
| Molecular Weight |
25,990 |
| Molar Extinction Coefficient |
15,840 |
| Isoelectric Point |
6.19 |
| Excitation |
485 nm |
| Emission |
535 nm |
Instructions for Use
| 1. |
The measurement of fluorescence is instrument-dependent. Please follow the
instructions for fluorescence measurements as described by the instrument manufacturer.
|
To determine the dose-response for a given application, prepare a dilution series
of the GFP in phosphate buffered saline pH 7.3. An example is shown on the right.
Material Safety Data
FOR RESEARCH USE ONLY. NOT INTENDED OR APPROVED
FOR HUMAN, DIAGNOSTICS OR VETERINARY
USE. Do not ingest, swallow or inhale. Do not get in
eyes, on skin, or on clothing. Wash thoroughly after
handling. For complete safety information see full
Material Safety Data Sheet.
References
| 1. |
Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov,
K. A., Lukyanov, S. A., and Matz, M. V. 2002. Diversity and evolution of
the green fluorescent protein family. Proc. Natl. Acad. Sci. USA.
99:4256-4261.
|
| 2. |
Bryan, B. J., Szent-Gyorgyi, C. 2001. Luciferases, fluorescent proteins,
nucleic acids encoding the luciferases and fluorescent proteins and
the use thereof in diagnostics, high throughput screening and
novelty items. U.S. Patent No. 6,232,107.
|
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