Technical Support

Renilla reniformis Green Fluorescent Protein Data Sheet

Catalog No. 0310

Product Description

Renilla reniformis (sea pansy) is a species of soft coral that is characterized by a green fluorescence. The green fluorescent protein obtained from this organism exhibits nearly symmetrical excitation and emission peaks with a relatively small Stokes shift.1 This is in contrast to the more commonly employed GFP from Aequorea victoria which has two wavelengths at which excitation occurs, and a broader emission spectra. The monomer extinction coefficient for Rr-GFP is about 5.5-fold higher than the Aequorea GFP which, in conjunction with a slightly higher quantum yield, produces a brighter fluorescence. Recombinant Renilla reniformis GFP has the same level of fluorescence as the native protein.2

Product Specifications
Protein 1mg (0310-1), 250µg (0310-2)
Long-Term Storage 2 years at - 80C
Aliquot to avoid repeated freezing and thawing.
Short-Term Storage 1 month at 4C
Formulations In: 10mM Na2HPO4, 140mM NaCl, 2 mM KH2PO4, 3mM KCl,
20% glycerol, pH 7.6
Molecular Weight 26kDa by SDS- PAGE
22kDa
Technical Information
Length 233 aa
Molecular Weight 25,990
Molar Extinction Coefficient 15,840
Isoelectric Point 6.19
Excitation 485 nm
Emission 535 nm

Instructions for Use

1. The measurement of fluorescence is instrument-dependent. Please follow the instructions for fluorescence measurements as described by the instrument manufacturer.

To determine the dose-response for a given application, prepare a dilution series of the GFP in phosphate buffered saline pH 7.3. An example is shown on the right.

Material Safety Data

FOR RESEARCH USE ONLY. NOT INTENDED OR APPROVED FOR HUMAN, DIAGNOSTICS OR VETERINARY USE. Do not ingest, swallow or inhale. Do not get in eyes, on skin, or on clothing. Wash thoroughly after handling. For complete safety information see full Material Safety Data Sheet.

References

1. Labas, Y. A., Gurskaya, N. G., Yanushevich, Y. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., and Matz, M. V. 2002. Diversity and evolution of the green fluorescent protein family. Proc. Natl. Acad. Sci. USA. 99:4256-4261.
2. Bryan, B. J., Szent-Gyorgyi, C. 2001. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items. U.S. Patent No. 6,232,107.